 |
 |
 |
 |
 |
 |
Sains Malaysiana 53(2)(2024): 249-265
http://doi.org/10.17576/jsm-2024-5302-02
Progeny Evaluation from the Crossing of Novelty-Type Phalaenopsis I Hsin Bee x Phalaenopsis pulcherrima var. champorensis
(Penilaian Progeni daripada Kacukan Jenis Kebaharuan Phalaenopsis I Hsin Bee x Phalaenopsis pulcherrima var. champorensis)
DEDEH SITI BADRIAH, DEWI PRAMANIK, SUSKANDARI
KARTIKANINGRUM, MINANGSARI DEWANTI, MAWADDAH, SURYAWATI, EKA FIBRIANTY, AGUS
MUHARAM* & KURNIAWAN BUDIARTO
Research
Center for Horticulture and Estate Crops, National Agency for Research and
Innovation, Cibinong Science Center, Jl. Raya Jakarta Bogor, Cibinong, Bogor
16915, Indonesia
Received: 6 June 2023/Accepted: 22 January 2024
Abstracts
The
floriculture industry has rapidly developed in Indonesia. One of the potential
genera of orchids is Phalaenopsis. New varieties must be developed to
compete with imported ones. Genetic improvement of Phalaenopsis has been
carried out through interspecific and intergeneric crossing - the crossing
between P. I Hsin Bee x P. pulcherrima var. champorensis is
dedicated to introduce harlequin type to P. pulcherrima. Forty progenies of
the first generation (F1) and their parents are used for the characterization and
evaluation. The results show that P. pulcherrima is more dominant (50%)
in flower shape and color based on the progeny composition. The introduction of
the harlequin type into P. pulcherrima is indicated by 35% of progenies
have dark colour at the flower and
matched with the spotted pattern of the female parent, indicating that
the female contributed partially to the respected traits. The progenies are divided into five distinct
groups based on nine qualitative characteristics. Three progenies, namely D829-4, D829-6, and D829-8, are considered
to have prominent features, such as more stable and symmetric coalescence of
red-brownish blotches on the flowers, representing harlequin type than other
progenies. These progenies will potentially be developed to be commercial
varieties.
Keywords: Harlequin;
hybridization; moon orchid; qualitative characters; quantitative
characters
Abstrak
Industri
florikultur telah berkembang pesat di Indonesia. Salah satu genus orkid yang berpotesi ialahPhalaenopsis. Varieti baharu daripada genus tersebut harus dikembangkan untuk menandingi varieti import. Penambahbaikan genetik Phalaenopsis telah dilakukan melalui kacukan antara spesies dan antara genus. Kacukan antara P. I Hsin x P. pulcherrima var. champorensis dilakukan untuk mengintegrasikan ciri harlequin pada P. pulcherrima. Sebanyak 40 progeni generasi pertama (F1) dan induk mereka telah digunakan untuk penilaian dan pencirian. Hasil menunjukkan bahwa P. pulcherrima lebih dominan (50%) dalam ciri bentuk dan warna bunga berdasarkan komposisi
progeni. Integrasi ciriharlequin ke
dalam P. pulcherrima ditunjukkan dengan banyaknya progeni (35%) yang mempunyai bunga berwarna
hitam yang sama dengan pola bercak pada induk betina. Sembilan ciri kualitatif yang membahagikan kesemuaprogeni menjadi
lima kumpulan, berdasarkan variasi warna dan bentuk daripada petal, sepal dorsal dan lateral, serta lobus-tengah. Tiga progeni, iaitu D829-4, D829-6
dan D829-8, menunjukkan ciri yang menonjol, seperti lebih stabil, gabungan
simetri tompok merah kecoklatan pada bunga yang memperlihatkan ciri harlequin berbanding dengan progeni
lain. Ketiga-tiga progeni tersebut
memiliki potensi untuk dikembangkan lebih lanjut menjadi varieti komersial.
Kata kunci: Ciri kualitatif; cirikuantitatif; harlequin; kacukan; orkid bulan
REFERENCES
Cai, J., Liu, X., Vanneste, K., Prost, S., Tsai,
W.C., Liu, K.W., Chen, L.J., He, Y., Xu, Q., Bian, C., Zheng, Z., Sun, F., Liu,
W., Hsiao, Y., Pan, Z.J., Hsu, C.C., Yang, Y.P., Hsu, Y.C., Chuang, Y.C. &
Liu, Z.J. 2015. The genome sequence of the orchid Phalaenopsis equestris. Nature Genetics 47(1): 65-72. https://doi.org/10.1038/ng.3149
Chen, C. 2020. The fundamental issue in the
phalaenopsis industry in the Netherlands. Journal of Agriculture and
Forestry 67(2): 89-100.
Chen, T.C., Lin, Y.Y. & Chen, W.H. 2020.
Breeding of Harlequin flowers in Phalaenopsis orchid. In Orchid
Biotechnology IV, edited by Chen, W.H. & Chen, H.H. World Scientific. pp. 15-41. https://doi.org/ doi:10.1142/9789811217777_0002
De, L. 2020. Morphological diversity in orchids. International
Journal of Botany Studies International 5(5): 299-238. https://doi.org/10.13140/RG.2.2.24041.31849
Dwiati, M., Susanto, A.H. & Prayoga, L. 2020. Intergeneric hybrids of Phalaenopsis 2166 x Vanda ‘Saint Valentine’ showing maternal inheritance: A genetic analysis based on the partial gene. Biodiversitas 21(11): 5138-5145. https://doi.org/10.13057/biodiv/d211119
Hartati, S. 2010. The
intergeneric crossing of Phalaenopsis sp. and Vanda tricolor. Journal
of Biotechnology and Biodiversity 1(1): 32-36.
Hsiao, Y.Y., Pan, Z.J., Hsu, C.C., Yang, Y.P., Hsu,
Y.C., Chuang, Y.C., Shih, H.H., Chen, W.H., Tsai, W.C. & Chen, H.H. 2011.
Research on orchid biology and biotechnology. Plant and Cell Physiology 52(9): 1467-1486. https://doi.org/10.1093/pcp/pcr100
Hsu, C.C., Chen, H.H. & Chen, W.H. 2018.
Phalaenopsis. In Ornamental Crops, Handbook of Plant Breeding 11, edited by van Hulenbroeck, J. Berlin
Heidelberg: Springer pp. 573-625.
https://doi.org/10.1007/978-3-319-90698-0
Hsu, C.C., Su, C.J., Jeng, M.F., Chen, W.H. &
Chen, H.H. 2019. A HORT1 retrotransposon insertion in the PeMYB11 promoter
causes harlequin/black flowers in Phalaenopsisorchids. Plant
Physiology 180(3): 1535-1548. https://doi.org/10.1104/pp.19.00205
Hsu, S.C., Cheng, T.C., Bolaños-Villegas, P., Chin,
S. W., & Chen, F. C. (2010). Pollen meiotic behavior about phalaenopsis
breeding. Acta Horticulturae 878: 139-144.
https://doi.org/10.17660/actahortic.2010.878.15
Hu, X., Lan, S., Song, X., Yang, F., Zhang, Z.,
Peng, D. & Ren, M. 2021. Genetic divergence between two sympatric ecotypes
of Phalaenopsis pulcherrima on Hainan Island. Diversity 13(9): 1-17. https://doi.org/10.3390/d13090446
Huang, J-Z., Lin, C-P., Cheng, T-C., Chang, B.C-H.,
Cheng, S-Y., Chen, Y-W., Lee, C-Y., Chin, S-W. & Chen, F-C. 2015. A de
novo floral transcriptome reveals clues into Phalaenopsis orchid flower
development. PLoS ONE 10(5): e0123474. https://doi.org/10.1371/journal.pone.0123474
Kassambara, A. & Mundt, F. 2020. Factoextra: Extract and visualize the results of multivariate
data analyses. CRAN.R-project.org.
Lee, Y.I. & Chung, M.C. 2020. Chromosome
analysis of Phalaenopsis Harlequin-type cultivars. In Orchid
Biotechnology IV. World Scientific. pp. 87-94. https://doi.org/ doi:10.1142/9789811217777_0005
Li, C., Dong, N., Zhao, Y., Wu, S., Liu, Z. &
Zhai, J. 2021. A review for the breeding of orchids: Current achievements and
prospects. Horticultural Plant Journal 7(5): 380-392.
https://doi.org/10.1016/j.hpj.2021.02.006
Liang, C.Y., Rengasamy, K.P., Huang, L.M., Hsu,
C.C., Jeng, M.F., Chen, W.H. & Chen, H.H. 2020. Assessment of violet-blue
color formation in Phalaenopsis orchids. BMC Plant Biology 20(1): 1-16.
https://doi.org/10.1186/s12870-020-02402-7
Lin, S., Lee, H.C., Chen, W.H., Chen, C.C., Kao,
Y.Y., Fu, Y.M., Chen, Y.H. & Lin, T.Y. 2001. Nuclear DNA contents of Phalaenopsis sp. and Doritis pulcherrima. Journal of the American Society for
Horticultural Science 126(2): 195-199. https://doi.org/ 10.21273/jashs.126.2.195
Liu, W.L., Shih, H.C., Weng, I.S., Ko, Y.Z., Tsai,
C.C., Chou, C.H. & Chiang, Y.C. 2016. Characterization of genomic
inheritance of intergeneric hybrids between Ascocenda and Phalaenopsis cultivars by GISH, PCR-RFLP, and RFLP. PLoS ONE 11(4): 1-14.
https://doi.org/10.1371/journal.pone.0153512
Marwoto, B., Badriah, D.S., Dewanti, M. &
Sanjaya, L. 2012. Persilangan interspesifik dan intergenerik anggrek Phalaenopsis untuk menghasilkan hibrid tipe baru. Prosiding Seminar Nasional Anggrek,
Makalah Pendukung 3: 101-116.
Meyer, N. 2010. Comparaison de variantes de
régressions logistiques PLS et de régression PLS sur variables qualitative: Application aux
données d’allélotypage [Comparing the linear and the logistic PLS
regression with qualitative predictors: Application to allelotyping data] (M. Maumy-Bertrand
& F. Bertrand, Trans.). Journal de La Société Française de Statistique 151(2): 1-18.
Nurmalinda, Kartikaningrum, S., Hayati, N.Q. & Widyastoety, D. 2011. Preferensi konsumen terhadap anggrek Phalaenopsis, Vanda, dan Dendrobium. Jurnal Hortikultura 21(4): 372. https://doi.org/10.21082/jhort.v21n4.2011.p372-384 Pramanik, D., Spaans, M., Kranenburg, T., Bogarin, D., Heijungs, R., Lens, F., Smets, E., & Gravendeel, B. 2022. Inflorescence lignification of natural species and horticultural hybrids of Phalaenopsis orchids. Scientiae Horticulturae 295:110845. https://doi.org/10.1016/j.scientia.2021.110845
R Team. 2019. RStudio: Integrated Development for
R. R Foundation for Statistical Computing.
R Team. 2016. R: A Language and Environment for
Statistical Computing. R Foundation for Statistical Computing.
Schrader, J., Shi, P., Royer, D.L., Peppe, D.J.,
Gallagher, R.V., Li, Y., Wang, R. & Wright, I.J. 2021. Leaf size estimation
based on leaf length, width and shape. Annals of Botany 128(4): 395-406.
https://doi.org/10.1093/aob/mcab078
Tang, C.Y. & Chen, W.H. 2007. Breeding and
development of new varieties in Phalaenopsis. In Orchid Biotechnology, edited by Chen, W.H. &
Chen, H.H. pp. 1-22. World Scientific Publishing Co. Ltd.
Tsai, C.C., Chiang, Y.C., Huang, S.C., Liu, W.L.
& Chou, C.H. 2010. Intergeneric hybridization, embryo rescue,
and molecular detection for intergeneric hybrids between Ascocenda and Phalaenopsis. Acta Horticulturae 829: 413-416.
https://doi.org/10.17660/actahortic.2009.829.67
van Tongerlo, E., van Ieperen, W., Dieleman, J.A.
& Marcelis, L.F.M. 2021. Vegetative traits can predict flowering quality in Phalaenopsis orchids despite large genotypic variation in response to
light and temperature. PLoS ONE 16: 1-22. https://doi.org/10.1371/journal.pone.0251405
Vo, T.C., Seo, J.W., Kim, C.K., Kim, H.Y. &
Lim, K.B. 2019. Breeding of the mini-type phalaenopsis cultivar ‘Yellow Green’,
having deep-yellow flowers with a red lip. Horticultural Science and
Technology 37(4): 540-547. https://doi.org/10.7235/ HORT.20190054
Watanabe, K.N. & Watanabe, J.A. 2000. Genetic
diversity and molecular genetics of ornamental plant species. Biotechnology
& Biotechnological Equipment 14(2): 19-21. https://doi.org/10.1080/13102818.2000.10819081
Yuan, S.C., Chin, S.W. & Chen, F.C. 2015.
Current trends of Phalaenopsis orchid breeding and study on pollen
storage. Acta Horticulturae 1078: 19-23. https://doi.org/ 10.17660/ActaHortic.2015.1078.1
Yukawa, T., Kita, K., Handa, T., Hidayat, T. &
Ito, M. 2005. Molecular phylogenetics of Phalaenopsis (Orchidaceae) and
allied genera: Re-evaluation of generic concepts. Acta Phytotaxonomica et
Geobotanica 56(2): 141-161.
Zhang, D., Zhao, X-W., Li, Y-Y., Ke, S-J., Yin,
W-L., Lan, S. & Liu, Z-J. 2022. Advances and prospects of orchid research
and industrialization. Horticulture Research 9: uhac220.
https://doi.org/10.1093/hr/uhac220
Zhang, Z., Gale, S.W., Li, J.H., Fischer, G.A.,
Ren, M.X. & Song, X.Q. 2019. Pollen-mediated gene flow ensures connectivity
among spatially discrete sub-populations of Phalaenopsis pulcherrima, a
tropical food-deceptive orchid. BMC Plant Biology 19(1): 1-16.
https://doi.org/10.1186/s12870-019-2179-y
*Corresponding
author; email: agus.muharam55@gmail.com
|
|||
|
